abstract：:The transmembrane inner nuclear membrane (INM) protein Samp1 is required for anchoring centrosomes near the nuclei. Using high-resolution fluorescence microscopy we show that Samp1 is distributed in a distinct and characteristic pattern in the nuclear envelope (NE), where it partially colocalizes with the LINC complex...

journal_title：Journal of cell science

authors： Gudise S,Figueroa RA,Lindberg R,Larsson V,Hallberg E

更新日期：2011-06-15 00:00:00

abstract：:IGFBP3 is a multi-functional protein that has IGF-dependent and IGF-independent actions in cultured cells. Here we show that the IGF binding domain (IBD), nuclear localization signal (NLS) and transactivation domain (TA) are conserved and functional in zebrafish Igfbp3. The in vivo roles of these domains were investig...

journal_title：Journal of cell science

authors： Zhong Y,Lu L,Zhou J,Li Y,Liu Y,Clemmons DR,Duan C

更新日期：2011-06-01 00:00:00

abstract：:Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2-GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics of single CUG-repe...

journal_title：Journal of cell science

authors： Querido E,Gallardo F,Beaudoin M,Ménard C,Chartrand P

更新日期：2011-05-15 00:00:00

abstract：:An intriguing question in cell biology is what targets proteins to, and regulates their translocation between, specific cellular locations. Here we report that the polybasic nuclear localization sequence (NLS) required for nuclear entry of the adapter protein and candidate human obesity gene product SH2B1β, also local...

journal_title：Journal of cell science

authors： Maures TJ,Su HW,Argetsinger LS,Grinstein S,Carter-Su C

更新日期：2011-05-01 00:00:00

abstract：:The notion that cell shape and spreading can regulate cell proliferation has evolved over several years, but only recently has this been linked to forces from within and upon the cell. This emerging area of mechanical signaling is proving to be wide-spread and important for all cell types. The microenvironment that su...

journal_title：Journal of cell science

authors： Provenzano PP,Keely PJ

更新日期：2011-04-15 00:00:00

abstract：:Dynamin-related GTPase proteins (DRPs) are main players in membrane remodelling. Conserved DRPs called mitofusins (Mfn1/Mfn2/Fzo1) mediate the fusion of mitochondrial outer membranes (OM). OM fusion depends on self-assembly and GTPase activity of mitofusins as well as on two other proteins, Ugo1 and Mdm30. Here, we de...

journal_title：Journal of cell science

authors： Anton F,Fres JM,Schauss A,Pinson B,Praefcke GJ,Langer T,Escobar-Henriques M

更新日期：2011-04-01 00:00:00

abstract：:Glutathione (GSH) levels progressively decline during aging and in neurodegenerative disorders. However, the contribution of such event in mediating neuronal cell death is still uncertain. In this report, we show that, in neuroblastoma cells as well as in primary mouse cortical neurons, GSH decrease, induced by buthio...

journal_title：Journal of cell science

authors： Aquilano K,Baldelli S,Cardaci S,Rotilio G,Ciriolo MR

更新日期：2011-04-01 00:00:00

abstract：:Cytokines, such as interferons, erythropoietin, leptin and most interleukins, signal through type 1 cytokine receptors and activate the canonical JAK-STAT pathway. Aberrant cytokine signalling underlies numerous pathologies and adequate, temporary receptor activation is therefore under tight control. Negative-feedback...

journal_title：Journal of cell science

authors： Wauman J,De Ceuninck L,Vanderroost N,Lievens S,Tavernier J

更新日期：2011-03-15 00:00:00

abstract：:Kindlin-2 is a FERM and PH domain-containing integrin-binding protein that is emerging as an important regulator of integrin activation. How kindlin-2 functions in integrin activation, however, is not known. We report here that kindlin-2 interacts with multiple phosphoinositides, preferentially with phosphatidylinosit...

journal_title：Journal of cell science

authors： Qu H,Tu Y,Shi X,Larjava H,Saleem MA,Shattil SJ,Fukuda K,Qin J,Kretzler M,Wu C

更新日期：2011-03-15 00:00:00

abstract：:Macroautophagy is a dynamic process whereby portions of the cytosol are encapsulated in double-membrane vesicles and delivered to the lysosome for degradation. Phosphatidylinositol-3-phosphate (PtdIns3P) is concentrated on autophagic vesicles and recruits effector proteins that are crucial for this process. The produc...

journal_title：Journal of cell science

authors： Martin KR,Xu Y,Looyenga BD,Davis RJ,Wu CL,Tremblay ML,Xu HE,MacKeigan JP

更新日期：2011-03-01 00:00:00

abstract：:The current paradigm of noncanonical NF-κB signaling suggests that the loss of TRAF2, TRAF3 or cIAP1 and cIAP2 leads to stabilization of NF-κB-inducing kinase (NIK) to activate the noncanonical pathway. Although a crucial role of RIP1 in the TNFα-induced canonical NF-κB pathway has been well established, its involveme...

journal_title：Journal of cell science

authors： Kim JY,Morgan M,Kim DG,Lee JY,Bai L,Lin Y,Liu ZG,Kim YS

更新日期：2011-02-15 00:00:00

abstract：:Circadian rhythms exist in most living organisms. The general molecular mechanisms that are used to generate 24-hour rhythms are conserved among organisms, although the details vary. These core clocks consist of multiple regulatory feedback loops, and must be coordinated and orchestrated appropriately for the fine-tun...

journal_title：Journal of cell science

authors： Kojima S,Shingle DL,Green CB

更新日期：2011-02-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that have important roles in gene regulation. We have previously reported that activin receptor-like kinase 7 (ALK7) and its ligand, Nodal, induce apoptosis in human epithelial ovarian cancer cells. In this study, we examined the regulation of ALK7 by miRNAs and demonstrate ...

journal_title：Journal of cell science

authors： Ye G,Fu G,Cui S,Zhao S,Bernaudo S,Bai Y,Ding Y,Zhang Y,Yang BB,Peng C

更新日期：2011-02-01 00:00:00

abstract：:TANGLED (TAN) is the founding member of a family of plant-specific proteins required for correct orientation of the division plane. Arabidopsis thaliana TAN is localized before prophase until the end of cytokinesis at the cortical division site (CDS), where it appears to help guide the cytokinetic apparatus towards th...

journal_title：Journal of cell science

authors： Rasmussen CG,Sun B,Smith LG

更新日期：2011-01-15 00:00:00

abstract：:TGFβ binding to the TGFβ receptor (TβR) activates R-Smad-dependent pathways, such as Smad2/3, and R-Smad-independent pathways, such as ERK1/2. The mechanism of the TGFβ-TβRII-TβRI-Smad2/3 pathway is established; however, it is not known how TGFβ activates ERK1/2. We show here that although TGFβ equally activated Smad2...

journal_title：Journal of cell science

authors： Bandyopadhyay B,Han A,Dai J,Fan J,Li Y,Chen M,Woodley DT,Li W

更新日期：2011-01-01 00:00:00

abstract：:Yeast Reb1 and its mammalian ortholog TTF1 are conserved Myb-type DNA-binding proteins that bind to specific sites near the 3'-end of rRNA genes (rDNA). Here, they participate in the termination of transcription driven by RNA polymerase I and block DNA replication forks approaching in the opposite direction. We found ...

journal_title：Journal of cell science

authors： Rodríguez-Sánchez L,Rodríguez-López M,García Z,Tenorio-Gómez M,Schvartzman JB,Krimer DB,Hernández P

更新日期：2011-01-01 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells is a pathological process that occurs during peritoneal dialysis. EMT leads to peritoneal fibrosis, ultrafiltration failure and eventually to the discontinuation of therapy. Signaling pathways involved in mesothelial EMT are thus of great inter...

journal_title：Journal of cell science

authors： Strippoli R,Benedicto I,Foronda M,Perez-Lozano ML,Sánchez-Perales S,López-Cabrera M,Del Pozo MÁ

更新日期：2010-12-15 00:00:00

abstract：:Increasing evidence indicates that cellular uptake of several molecules can occur independently of functional dynamin, but the molecular players that regulate dynamin-independent endocytosis and the subsequent trafficking steps are still largely unknown. A survival-based short-hairpin (sh) RNA screen using a cell line...

journal_title：Journal of cell science

authors： Krag C,Malmberg EK,Salcini AE

更新日期：2010-12-15 00:00:00

abstract：:The existence of two forms of the chromosome passenger complex (CPC) in the mammalian oocyte has meant that its role in female meiosis has remained unclear. Here we use loss- and gain-of function approaches to assess the meiotic functions of one of the shared components of these complexes, INCENP, and of the variable ...

journal_title：Journal of cell science

authors： Sharif B,Na J,Lykke-Hartmann K,McLaughlin SH,Laue E,Glover DM,Zernicka-Goetz M

更新日期：2010-12-15 00:00:00

abstract：:Naegleria gruberi is a single-celled eukaryote best known for its remarkable ability to form an entire microtubule cytoskeleton de novo during its metamorphosis from an amoeba into a flagellate, including basal bodies (equivalent to centrioles), flagella and a cytoplasmic microtubule array. Our publicly available full...

journal_title：Journal of cell science

authors： Fritz-Laylin LK,Cande WZ

更新日期：2010-12-01 00:00:00

abstract：:Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...

journal_title：Journal of cell science

authors： Blumbach K,Zweers MC,Brunner G,Peters AS,Schmitz M,Schulz JN,Schild A,Denton CP,Sakai T,Fässler R,Krieg T,Eckes B

更新日期：2010-11-15 00:00:00

abstract：:Circadian rhythms regulate a wide variety of physiological and metabolic processes. The clock machinery comprises complex transcriptional-translational feedback loops that, through the action of specific transcription factors, modulate the expression of as many as 10% of cellular transcripts. This marked change in gen...

journal_title：Journal of cell science

authors： Bellet MM,Sassone-Corsi P

更新日期：2010-11-15 00:00:00

abstract：:Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character of the such complexes are enigmatic. We use steric-exclusion chromatography, affinity pull-...

journal_title：Journal of cell science

authors： Yokoyama N,Golebiewska U,Wang HY,Malbon CC

更新日期：2010-11-01 00:00:00

abstract：:Dynamins are a conserved family of proteins involved in membrane fusion and fission. Although mammalian dynamins are known to be involved in several membrane-trafficking events, the role of dynamin-1 in endocytosis is the best-characterised role of this protein family. Despite many similarities between endocytosis in ...

journal_title：Journal of cell science

authors： Smaczynska-de Rooij II,Allwood EG,Aghamohammadzadeh S,Hettema EH,Goldberg MW,Ayscough KR

更新日期：2010-10-15 00:00:00

abstract：:Microtubules--polymers of tubulin--perform essential functions, including regulation of cell shape, intracellular transport and cell motility. How microtubules are adapted to perform multiple diverse functions is not well understood. Post-translational modifications of tubulin subunits diversify the outer and luminal ...

journal_title：Journal of cell science

authors： Wloga D,Gaertig J

更新日期：2010-10-15 00:00:00

abstract：:We previously showed that the cell-cell junction protein plakoglobin (PG) not only suppresses motility of keratinocytes in contact with each other, but also, unexpectedly, of single cells. Here we show that PG deficiency results in extracellular matrix (ECM)-dependent disruption of mature focal adhesions and cortical ...

journal_title：Journal of cell science

authors： Todorović V,Desai BV,Patterson MJ,Amargo EV,Dubash AD,Yin T,Jones JC,Green KJ

更新日期：2010-10-15 00:00:00

abstract：:We investigated the link between cell movement and plasma membrane recycling using a fast-acting, temperature-sensitive mutant of the Dictyostelium SecA exocytic protein. Strikingly, most mutant cells become almost paralysed within minutes at the restrictive temperature. However, they can still sense cyclic-AMP (cAMP)...

journal_title：Journal of cell science

authors： Zanchi R,Howard G,Bretscher MS,Kay RR

更新日期：2010-10-01 00:00:00

abstract：:AMP-activated protein kinase (AMPK), a cellular metabolic sensor, is essential in energy regulation and metabolism. Hepatocyte polarization during liver development and regeneration parallels increased metabolism. The current study investigates the effects of AMPK and its upstream activator LKB1 on polarity and bile c...

journal_title：Journal of cell science

authors： Fu D,Wakabayashi Y,Ido Y,Lippincott-Schwartz J,Arias IM

更新日期：2010-10-01 00:00:00

abstract：:Interactions between melanocytes and neighboring cells in the skin are important in regulating skin color in humans. We recently demonstrated that the less pigmented and thicker skin on the palms and soles is regulated by underlying fibroblasts in those areas, specifically via a secreted factor (DKK1) that modulates W...

journal_title：Journal of cell science

authors： Choi W,Wolber R,Gerwat W,Mann T,Batzer J,Smuda C,Liu H,Kolbe L,Hearing VJ

更新日期：2010-09-15 00:00:00

abstract：:The mucolipin (TRPML) subfamily of transient receptor potential (TRP) cation channels consists of three members that play various roles in the regulation of membrane and protein sorting along endo-lysosomal pathways. Loss-of-function mutations in TRPML1 cause the neurodegenerative lysosomal storage disorder, mucolipid...

journal_title：Journal of cell science

authors： Zeevi DA,Lev S,Frumkin A,Minke B,Bach G

更新日期：2010-09-15 00:00:00

abstract：:An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue injury. Lumican, an extracellular matrix component belonging to the small leucine rich proteoglycan (SLRP) family, is one of the major keratan sulfate proteoglycans (KSPGs) within the corneal stroma. Increasing ev...

journal_title：Journal of cell science

authors： Hayashi Y,Call MK,Chikama T,Liu H,Carlson EC,Sun Y,Pearlman E,Funderburgh JL,Babcock G,Liu CY,Ohashi Y,Kao WW

更新日期：2010-09-01 00:00:00

abstract：:Myogenic differentiation in adult muscle is normally suppressed and can be activated by myogenic cues in a subset of activated satellite cells. The switch mechanism that turns myogenesis on and off is not defined. In the present study, we demonstrate that tissue inhibitor of metalloproteinase 3 (TIMP3), the endogenous...

journal_title：Journal of cell science

authors： Liu H,Chen SE,Jin B,Carson JA,Niu A,Durham W,Lai JY,Li YP

更新日期：2010-09-01 00:00:00

abstract：:CDC14 was originally identified by L. Hartwell in his famous screen for genes that regulate the budding yeast cell cycle. Subsequent work showed that Cdc14 belongs to a family of highly conserved dual-specificity phosphatases that are present in a wide range of organisms from yeast to human. Human CDC14B is even able ...

journal_title：Journal of cell science

authors： Mocciaro A,Schiebel E

更新日期：2010-09-01 00:00:00

abstract：:Using a genome-wide technical knockout, we isolated a newly identified set of GRIM (genes associated with retinoid-interferon-induced mortality) genes; GRIM genes mediate IFN- and retinoic-acid (RA)-induced cell death. Here, we describe the isolation and characterization of one such gene, GRIM-1. Three proteins, with ...

journal_title：Journal of cell science

authors： Hofmann ER,Nallar SC,Lin L,D'Cunha J,Lindner DJ,Weihua X,Kalvakolanu DV

更新日期：2010-08-15 00:00:00

abstract：:Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to...

journal_title：Journal of cell science

authors： Boeckeler K,Rosse C,Howell M,Parker PJ

更新日期：2010-08-15 00:00:00

abstract：:p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction ...

journal_title：Journal of cell science

authors： Casagolda D,Del Valle-Pérez B,Valls G,Lugilde E,Vinyoles M,Casado-Vela J,Solanas G,Batlle E,Reynolds AB,Casal JI,de Herreros AG,Duñach M

更新日期：2010-08-01 00:00:00

abstract：:RINGO/Speedy proteins are direct activators of Cdk1 and Cdk2 that have no sequence homology to cyclins. We have characterized the role in cell-cycle progression of a new human member of this protein family referred to as RINGO C. We show that siRNA-mediated knockdown of RINGO C results in premature mitotic exit with m...

journal_title：Journal of cell science

authors： Mourón S,de Cárcer G,Seco E,Fernández-Miranda G,Malumbres M,Nebreda AR

更新日期：2010-08-01 00:00:00

abstract：:Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly ...

journal_title：Journal of cell science

authors： Lone M,Kungl T,Koper A,Bottenberg W,Kammerer R,Klein M,Sweeney ST,Auburn RP,O'Kane CJ,Prokop A

更新日期：2010-07-15 00:00:00

abstract：:Ferlin proteins mediate membrane-fusion events in response to Ca(2+). Myoferlin, a member of the ferlin family, is required for normal muscle development, during which it mediates myoblast fusion. We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection...

journal_title：Journal of cell science

authors： Demonbreun AR,Lapidos KA,Heretis K,Levin S,Dale R,Pytel P,Svensson EC,McNally EM

更新日期：2010-07-15 00:00:00

abstract：:Fatty acids constitute an important energy source for various tissues. The mechanisms that mediate and control uptake of free fatty acids from the circulation, however, are poorly understood. Here we show that efficient fatty-acid uptake by yeast cells requires the protein kinase Ypk1, the orthologue of the human seru...

journal_title：Journal of cell science

authors： Jacquier N,Schneiter R

更新日期：2010-07-01 00:00:00